Ted cellsorting analysis. Description of the results of SPC-01 cells FACS analysis. Additional file 3: Figure S1. Fluorescence-activated cell-sorting profiles of pluripotent and neural markers in SPC-01 human fetal neural stem cells. Additional file 4: Figure S2. Expression of early neural and glial markers by SPC-01 cells 8 weeks after transplantation into SCI. Eight weeks after transplantation, SPC-01 cells expressed the early neural markers NSE (S2A and B) and nestin (S2C), the early oligodendroglial marker Olig2 (S2D), and the astroglial marker GFAP (S2E). Seventeen weeks after transplantation, SPC-01 cells were positive for CNPase (S2F). Additional file 5: Figure S3. Orthogonal projection for Figure 6 images. Abbreviations 4-OHT: 4-Hydroxy-tamoxifen; A1ctb: actin beta; BBB test: Basso, Beattie, and Bresnahan test; BDNF: brain-derived neurotrophic factor; CGRP: calcitonin gene-related peptide; ChAT: choline acetyltransferase; CNPase: 2,3-cyclic nucleotide 3-phosphodiesterase; Cq: cycle of quantification; DMEM: F12: Dulbecco modified Eagle medium, nutrient mixture F-12; DNA: desoxyribonucleic acid; EGF: epidermal growth factor; FGF: fibroblast growth factor; GABA: -aminobutyric acid; GAP43: growth-associated protein 43; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; GFAP: glial fibrillary acidic protein; GFP: green fluorescent protein; HB9: homeobox 9; HBSS: HankAmemori et al. Stem Cell Research Therapy 2013, 4:68 http://stemcellres.com/content/4/3/Page 15 of10. Hofstetter CP, Schwarz EJ, Hess D, Widenfalk J, El Manira A, Prockop DJ, Olson L: Marrow stromal cells form guiding strands in the injured spinal cord and promote recovery. Proc Natl Acad Sci U S A 2002, 99:2199?204. 11. Urdzikova L, Jendelova P, Glogarova K, Burian M, Hajek M, Sykova E: Transplantation of bone marrow stem cells as well as mobilization by granulocyte-colony stimulating factor promotes recovery after spinal cord injury in rats. J Neurotrauma 2006, 23:1379?391. 12. Amemori T, Jendelova P, Ruzickova K, Arboleda D, Sykova E: Co-transplantation of olfactory ensheathing glia and mesenchymal stromal cells does not have synergistic effects after spinal cord injury in the rat. Cytotherapy 2010, 12:212?25. 13. Cummings BJ, Uchida N, Tamaki SJ, Salazar DL, Hooshmand M, Summers R, Gage FH, Anderson AJ: Human neural stem cells differentiate and promote locomotor recovery in spinal cord-injured mice. Proc Natl Acad Sci U S A 2005, 102:14069?4074. 14. Hooshmand Nelfinavir (Mesylate) MJ, Sontag CJ, Uchida N, Tamaki S, Anderson AJ, Cummings BJ: Analysis of host-mediated repair mechanisms after human CNS-stem cell transplantation for spinal cord PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/16989806 injury: correlation of engraftment with recovery. PLoS One 2009, 4:e5871. 15. Yan J, Xu L, Welsh AM, Hatfield G, Hazel T, Johe K, Koliatsos VE: Extensive neuronal differentiation of human neural stem cell grafts in adult rat spinal cord. PLoS Med 2007, 4:e39. 16. Yasuda A, Tsuji O, Shibata S, Nori S, Takano M, Kobayashi Y, Takahashi Y, Fujiyoshi K, Hara CM, Miyawaki A, Okano HJ, Toyama Y, Nakamura M, Okano H: Significance of remyelination by neural stem/progenitor cells transplanted into the injured spinal cord. Stem Cells 2011, 29:1983?994. 17. Lee SH, Chung YN, Kim YH, Kim YJ, Park JP, Kwon DK, Kwon OS, Heo JH, Ryu S, Kang HJ, Paek SH, Wang KC, Kim SU, Yoon BW: Effects of human neural stem cell transplantation in canine spinal cord hemisection. Neurol Res 2009, 31:996?002. 18. Pollock K, Stroemer P, Patel S, Stevanato L, Hope A, Miljan E, Dong Z, Hodg.