A F, Luchetti M, Spadoni T, Mancini M, Fraticelli P, Sambo P, Funaro A, Kazlauskas A, Avvedimento EV, Gabrielli A: Stimulatory autoantibodies to the PDGF receptor in systemic sclerosis. N Engl J Med 2006, 354:2667-2676. 6. Tamby MC, Servettaz A, Tamas N, Reinbolt J, Caux F, Meyer O, Allanore Y, Kahan A, Guillevin L, Mouthon L: IgG from patients with systemic sclerosis bind to DNA antitopoisomerase 1 in normal human fibroblasts extracts. Biologics 2008, 2:583-591. 7. Ihn H, Sato S, Fujimoto M, Igarashi A, Yazawa N, Kubo M, Kikuchi K, Takehara K, Tamaki K: Characterization of autoantibodies to endothelial cells in systemic sclerosis (SSc): association with pulmonary fibrosis. Clin Exp Immunol 2000, 119:203-209. 8. Garc de la Pe -Lefebvre P, Chanseaud Y, Tamby MC, Reinbolt J, Batteux F, Allanore Y, Kahan A, Meyer O, Benveniste O, Boyer O, Guillevin L, Boissier MC, Mouthon L: IgG reactivity with a 100-kDa tissue and endothelial cell antigen identified as topoisomerase 1 distinguishes between limited and diffuse systemic sclerosis patients. Clin Immunol 2004, 111:241-251. 9. Mihai C, Tervaert JW: Anti-endothelial cell antibodies in systemic sclerosis. Ann Rheum Dis 2010, 69:319-324. 10. Terrier B, Tamby MC, Camoin L, Guilpain P, Broussard C, Bussone G, Yaici A, Hotellier F, Simonneau G, Guillevin L, Humbert M, Mouthon L:Abbreviations 2-DE: two-dimensional electrophoresis; ACA: anti-centromere antibody; ANA: antinuclear antibodies; ATA: anti-topoisomerase I antibody; dcSSc: diffuse cutaneous systemic sclerosis; EDTA: ethylenediaminetetraacetic acid; EGTA: ethyleneglycoltetraacetic acid; HC: healthy controls; hnRNP L: heterogeneous nuclear ribonucleoprotein L; ILD: interstitial lung disease; lcSSc: limited cutaneous systemic sclerosis; MALDI: matrix-assisted PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/6833145 laser desorption/ ionization; MS: mass spectrometry; MS/MS: tandem mass spectrometry; PAH: pulmonary arterial hypertension; PDGF: platelet-derived growth factor; PRDX2: peroxiredoxin 2; PVDF: polyvinylidene difluoride; ROS: reactive oxygen species; SLE: systemic lupus erythematosus; SOD2: superoxide dismutase mitochondrial precursor; SRC: scleroderma renal crisis; SSc: systemic sclerosis; TGF: transforming growth factor; TOF: time of flight; TPI: triosephosphate isomerase. Acknowledgements GB received financial support from Avenir Mutualiste des Professions Lib ales Ind endantes (AMPLI), the Soci ?Nationale Fran ise de M ecine Interne, the Fonds d'Etudes et de Recherche du Corps M ical des h itaux de Paris and the Direction R ionale des Affaires Sanitaires et Sociales d'Ile-de-France. HD received financial support from AMPLI and Association pour la Recherche en M ecine Interne et en Immunologie Clinique (ARMIIC). MCT received a grant from Pfizer and from the Direction de la Recherche Clinique from the Assistance Publique-H itaux de Paris (Programme Hospitalier de Recherche Clinique National: Auto-Hypertension Art ielle Pulmonaire (Auto-HTAP). We thank Pfizer and the Direction de laBussone et al. Arthritis Research Therapy 2011, 13:R74 http://arthritis-research.com/content/13/3/RPage 12 of11.12.13.14.15. 16. 17.18.19. 20.21.22.23.24.25.26.27.Identification of target antigens of antifibroblast antibodies in pulmonary Capecitabine arterial hypertension. Am J Respir Crit Care Med 2008, 177:1128-1134. Servettaz A, Guilpain P, Camoin L, Mayeux P, Broussard C, Tamby MC, Tamas N, Kaveri SV, Guillevin L, Mouthon L: Identification of target antigens of antiendothelial cell antibodies in healthy individuals:.